1. For checking a pair of primers, the valid primer sequence format:

Right example 1:

TGTCCTGTTCTCCAGGCTCT

Right example 2:

5'-TGTCCTGTTCTCCAGGCTCT-3'

Right example 3:

5TGTCCTGTTCTCCAGGCTCT3

Wrong example (No odd letters are allowed in the sequence):

TGTCCTGTTC666TCCAGGCTCT

2. For checking multiple primers, the FASTA format is required:

Note: Please make sure that your sequences have different names as MFEprimer use these names to distinguish the sequences.